Characterization of crystals of small ribosomal subunits.
نویسندگان
چکیده
Crystals of intact small ribosomal subunits from Thermus thermophilus have been obtained from functionally active particles. The crystals (P42(1)2, 407 A x 407 A x 171 A) are suitable for X-ray crystallography analysis to 9.9 A using synchrotron radiation at cryotemperature. Crystallographic data from native and a potential heavy-atom derivative have been collected.
منابع مشابه
Characterization and preliminary crystallographic studies on large ribosomal subunits from Thermus thermophilus.
Diffracting crystals, suitable for X-ray crystallographic analysis, have been obtained from large (50 S) ribosomal subunits from Thermus thermophilus. These crystals, with P4(1)2(1)2 symmetry and a unit cell of 495 A x 495 A x 196 A, reach typically a size of 0.15 mm x 0.25 mm x 0.35 mm. Using synchrotron radiation at cryo-temperature, these crystals diffract X-rays to better than 9 A resolutio...
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ورودعنوان ژورنال:
- Journal of molecular biology
دوره 203 3 شماره
صفحات -
تاریخ انتشار 1988